Outcomes

After completion of the course the student should be able to

1

Bio molecules

CO 1 : Gives brief knowledge about the significance of water, buffer and analytical techniques used to detect the quality of water and soil.

CO 2 : Introduces carbohydrates, its classification, properties and biological functions.

CO 3 : Provides detailed information about the different types of lipids, their properties and biological significance in living organisms.

CO 4 : Teaches in depth knowledge about proteins, different levels of their structural organization and functions.

CO 5 : Establishes the fundamentals of Nucleic acids and porphyrins.

Practical – Qualitative analysis

●       Gives the expertise to the student in the preparation of buffers and steps involved in determining the pH of a given solution.

●       Develop skills in students to analyses and identify different bio molecules qualitatively.

●       Introduces what is absorption maxima and how to determine absorption maxima of p-Nitro phenol and methyl orange.

●       Teaches the basics of absorption spectrum and how to record absorption spectra of BSA and DNA.

II

Analytical Techniques

CO 1 : Introduces various analytical techniques involved in tissues homogenization and fractionation.

CO 2 : Provides fundamental knowledge about chromatographic technique, its types, principle involved in it and its applications in the separation of bio molecules.

CO 3 : Teaches the basic principles of various spectroscopic and tracer techniques and their applications in elucidating the structure of bio molecules, and biochemical pathways.

CO 4 : Gains knowledge about the fundamentals of electrophoresis and its applications in separation of biomolecules like proteins, lipoproteins and nucleic acids.

CO 5 : Get knowledge about basic microbial techniques involved in isolation, purification and biochemical characterization of microbes and their preservation.

II

Practical – Biochemical techniques

●       Generates the expertise to the student for isolation of nucleic acids and their identification.

●       Develops the skill to isolate egg albumin, cholesterol and polysaccharides from their respective sources.

●       Creates the ability to separate different amino acids using paper chromatography

●       Gains proficiency to separate plasma proteins using electrophoretic technique.

III

Enzymology, Bioenergetics and Intermediary metabolism

CO 1 : Introduces enzymes, its classification and nomenclature, mechanism of enzyme action and its applications in diagnostic labs and various industries.

CO 2 : Teaches the basic principles of thermodynamics, its relevance in the formation and maintenance of ATP and mechanism of oxidative phosphorylation.

CO 3 : Gains knowledge about how the carbohydrates get metabolized for the purpose of energy and other physiological functions in the body.

CO 4 : Enable the student to understand the metabolic fate of lipids and inborn errors associated with it.

CO 5 : Gives knowledge about the metabolism of amino acids, significance of urea cycle and various metabolic disorders like phenyl ketonuria, alkaptonuria etc.,

Practical – Quantitative Analysis

●     Provide the expertise for quantification of various enzymes.

●     Develops skill to estimate glucose, proteins and lipid levels in blood quantitatively.

●     Cultivate ability to find out optimum parameter for a particular enzyme.

IV

Physiology, Nutritional and Clinical Biochemistry

●       Get knowledge about digestion and absorption of carbohydrates, Proteins and lipids, composition of blood, its biological relevance and clinical significance.

●       Introduces the anatomy, structure and function of nervous and excretory systems.

●       Teaches the basics of endocrinology, secretion of different hormones, mechanism of their action and their role in maintaining the homeostasis of cells.

●       Understand what is balanced diet, its importance in keeping healthy body, biological role of vitamins and diseases. associated with its deficiency.

●       Gains knowledge about the clinical significance of isozymes, plasma proteins and other enzyme markers in organ functioning.

IV

Practical – Nutritional and clinical biochemistry

●       Creates ability to quantify minerals like calcium, iron and vitamin C.

●       Train the student to quantify hemoglobin, total WBC and RBC in blood and interpret the results.

●       Clinical chemistry unit along with practical will enable the student to do diagnostic tests for liver diseases, Gastro intestinal diseases, renal diseases and nutritional deficiencies.

IV

Microbiology, Immunology and Molecular Biology

CO 1 : Enable the student to know numerous microorganisms, including bacteria, fungus, and viruses, as well as their structures, other characteristics, and the diseases they cause..

CO 2 : Introduces nitrogen cycle, different ways of nitrogen fixation, its importance in the synthesis of glutamine and its regulation.

CO 3 : Teaches the basics of fermentation technology, its types and its commercial applications in the production of alcohol, acids, solvents and antibiotics.

CO 4 : Develops understanding of the immune system, vaccines, and the a etiology of immune deficiency disorders and auto immune diseases.

CO 5 : Teaches basics of the central dogma of the cell, outlines cloning technologies and its applications in agriculture, medicine and other industries.

IV

Practical – Microbiology and Immunology

●       Inculcate the knowledge about safer handling of bio- samples and GLPs.

●       Gives training to the students to prepare microbiological media and sterilize it.

●       Enable the student to become expert in growing the respective culture for the production of appropriate metabolites.

●       The practical will provide the expertise to the student to work in microbiology laboratory, food and pharma industries, and biotech companies for production of vaccines and other life saving drugs

V

SEC-Clinical Biochemistry

CO 1 :     Understand the organization of clinical laboratory, various instruments used in it, significance of automation, types of specimens, safety regulations and quality control.

CO 2 :     Know the various enzymes involved in normal functioning of liver as well as kidney and their clinical relevance.

CO 3 :     Creates knowledge about the digestion, absorption, assimilation of glucose, Hormonal regulation of glucose and the metabolic disorders associated with it.

CO 4 :     Introduces the different types of lipids, lipoproteins, their metabolic fate, physiological role and clinical significance.

CO 5 :     Provides basics of cardiovascular physiology, biochemical symptoms associated with various heart diseases and various isozymes and their levels in the disease diagnosis.

V

Practical – Clinical Biochemistry

Provides expertise

●         in collection of blood

●       Principle involved in the Separation of plasma and serum

●       Quantitative estimation of clinically relevant bio molecules like glucose triglycerides, cholesterol, tropon in and lipoproteins

●       Quantification of enzymes like ALT, AST, CK, LDH

●       Understand the clinical significance of the levels of various bio molecules and enzyme markers.

V

SEC-

Haematological and Immunological techniques

CO 1 : Understand the organization of immunological laboratory and its maintenance, significance of normal range, reference values, internal and external standards, WHO standards and quality control.

CO 2 : Learn different components of blood and their functions

CO 3 : Get knowledge on advance molecular diagnostic tools like ELISA, RT-PCR microtome sections and histopathology

CO 4 : Fundamentals of Auto-immunity and its classification with examples

CO 5 : Basics of immunoglobulin, types, structure, its function and  production of antibodies.

Creates expertise in

●       Determining blood group based on Hem agglutination test

●       Estimating total RBC and WBC count and its significance in diagnosing the clinical manifestations

●       Determining ESR, PCV, Mean cell RBC volume and Hemoglobin content

●       Performing immunochemical techniques to diagnose various auto-immune disorders and microbial infections.